Translational_Unit

Part:BBa_K3045002:Design

Designed by: Sarah García Hualde   Group: iGEM19_Navarra_BG   (2019-09-10)


pGBSS-Xa-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 392
    Illegal BglII site found at 1138
    Illegal BglII site found at 1601
    Illegal BglII site found at 1640
    Illegal BamHI site found at 1976
    Illegal BamHI site found at 3037
    Illegal XhoI site found at 1342
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4015
    Illegal BsaI.rc site found at 4302


Design Notes

The plasmid was constructed using GoldenBraid 3.0 (https://gbcloning.upv.es/)


Source

GBSS: Granule-bound starch synthase cDNA from Arabidopsis thaliana Factor Xa cleavage site: Ile-Glu-Gly-Arg GFP: Green fluoresce protein eGFP


References